InCell 1000 GE lifesciences High-content screening unit.
InCell 1000 summary
IN Cell Analyzer 1000 is a flexible, modular, cellular and subcellular imaging system for fast, automated imaging in fixed and live cells.
This unit is an automated epifluorescence-based microscope with motorised stage, laser autofocus and image acquisition software. It uses a Nikon microscope and optics with a xenon light source and a range of Chroma excitation/emission filters as well as Chroma multi-pass dichroics. The unit in the DDBL also has temperature control, automated liquid handling, a transmitted light unit, microscope slide imaging and an optical Z-sectioning module.
This unit is primarily designed for high-content, high throughput assays. It is well suited to image capture from multi well plates (up to 96 well). Although it does provide excellent epiflourescent image quality it is not designed primarily for capturing publication quality images or for quickly looking at samples to assess staining. These tasks are better done on microscopes designed for this purpose.
Using the InCell 1000
Monash users outside the DDBL may use the InCell 1000, however you will need to be trained and registered. You will need to be trained on use of both the InCell 1000 itself as well as the accompanying In Cell investigator software. The InCell 1000 is located in building 13E (Pharmacology).
If you would like to arrange training for the InCell 1000 you will need to contact either Carline Hick: firstname.lastname@example.org
or Sebastian Furness: email@example.com
Phone: 9905 8194
Registered users will be able to book time on the InCell 1000 using either Netscape calendar or the Oracle client.
For those familiar with these calendar systems the InCell 1000 is listed as a resource with the following name: res:Med ddbl InCell 1000. Please note that you MUST include an email to both Sebastian Furness and Caroline Hick for your booking to be active.
For those users unfamiliar with this system please follow this link for a full description Booking the InCell 1000.doc
Hardware and software training $75/Hr
Assisted use $75/Hr
Unassisted use (experienced users) $30/Hr
Offline data analysis No charge but by negotiation (see below)
InCell 1000 objectives
4x, 10x, 20x and 40x objectives are available.
InCell 1000 filters and dichroics
Filters are listed with transmission maxima followed by transmission bandwidth.
Excitation Emission Example dye
360/40 460/40 DAPI, Hoecsht
480/40 535/50 FITC, GFP
405/20 585/40 GFP2
565/30 620/60 Texas Red
620/60 700/75 Cy5
Note that the excitation and emission filters can be selected independently, therefore it is possible to accommodate conjugated dyes and FRET style experiments.
Dichroics are listed with suggested dye combinations and a link to the Chroma website which proves information on the spectral characteristics of these dichroics.
Dichroic Example Dyes Link
61000v2bs DAPI/FITC/Cy3 http://www.chroma.com/index.php?option=com_products&Itemid=53&task=details&productType=part&id=113
61002bs DAPI/FITC/Texas Red http://www.chroma.com/index.php?option=com_products&Itemid=53&task=details&productType=part&id=119
61003bs DAPI/FITC/Cy5 http://www.chroma.com/index.php?option=com_products&Itemid=53&task=details&productType=part&id=122
88100bs DAPI/FITC/Texas Red/Cy5 http://www.chroma.com/index.php?option=com_products&Itemid=53&task=details&productType=part&id=199
61004bs DAPI/Cy3/Cy5 http://www.chroma.com/index.php?option=com_products&Itemid=53&task=details&productType=part&id=125
InCell software support
In Cell investigator software is available for data captured on the InCell 1000. Presently we have 2 licences for this software. Data sets acquired on the InCell 1000 are typically very large and can take many hours for the software to analyse. Users will therefore have to negotiate with Caroline or Sebastian for use of computer time to perform data analysis or alternatively purchase their own copy of the s