Proteomics has matured as a discipline and emerged as a key technology in life science and medical research. It involves the study of protein expression with unprecedented precision and accuracy. Performed at a global level, the discovery, quantitation and analysis of post-translational modifications of thousands of proteins can be performed simultaneously. Alternatively, the use of targeted proteomics techniques can afford extensive coverage of a single pathway or pathways of interest. These technologies can be applied to multiple sample types from whole tissues to sorted cell populations and analysed in conjunction with several separation and quantitative platforms including multiple dimensions of chromatographic separation, 1D/2D gel separations, off gel fractionation, SILAC and iTRAQ stable isotope labelling strategies.
The Department has recently established a strong research proteomics focus with several groups now working with cutting edge instrumentation to address all areas of biochemistry and molecular biology. These include quantitative proteomics, the analysis of a range of post-translational modifications, immunoproteomics, peptide biology, protease biology and structural proteomics.
Professor Tony Purcell (immunoproteomics, quantitative proteomics)
Professor Ian Smith (peptide biology)
Professor Ed Nice (biomarker discovery)
Dr Oded Kleifeld (protease biology, quantitative proteomics)
Dr Nadine Dudek (autoantigen epitope discovery)
Dr Nathan Croft (viral epitope discovery and quantitation)
Dr Ralf Schittenhelm (bacterial epitope discovery)
Dr David Steer (core proteomics facility manager)
Dr Bosco Ho (bioinformatics)
- AB SCIEX 5600 Plus TripleToF LC-MS (High end proteomic and peptidomic analysis, quantitation, next generation data independent acquisition (SWATH))
- AB SCIEX 5500 Q-TRAP LC-MS (quantitation and targeted mass spectrometry)
- Agilent Triple Quadrupole LC-MS (quantitation and targeted mass spectrometry)
- AB SCIEX SelexION ion mobility LC-MS (separation of peptides and proteins based on mass and shape/conformation)
- Thermo Scientific Q-Exactive Q-Orbitrap LC-MS (High end proteomic and peptidomic analysis, quantitation)
- AB SCIEX 4700 MALDI-TOF/TOF (Protein identification)
- Bruker MicroQ Q-TOF LC-MS (general proteomics)
- Bruker HCT ion Trap LC-MS (general proteomics)
- Procise Edman Sequencer (N-terminal sequence analysis)
Access to these capabilities is available through collaboration with any of the named investigators and/or on a fee for service in the Biomedical Proteomics Facility.