Introduction:
The production and biophysical analysis of proteins are key technologies in the biomedical sciences. The first step in being able to experimentally study the structure and function of a protein is being able to recombinantly produce the protein and analyse its physical characteristics. The Department possesses a range of cutting-edge technologies, funded by grants from the ARC, NHMRC, Faculty of Medicine and the Department that allow for the production of recombinant proteins and their analysis. These technologies include the high-throughput production of recombinant proteins from either bacterial or mammalian cells, spectroscopic analysis of a protein's structure, stability and function using circular dichroism spectroscopy, fluorescence spectroscopy, isothermal calorimetry and dynamic light scattering.
The Department has an exceptionally strong research focus in the area of protein structure and function and many groups use the equipment to address their research questions. The equipment can also be accessed through collaboration and/or on a fee-for-service in the Protein Production facility.
Key Personnel:
Professor Stephen Bottomley (protein production and biophyscial analysis of proteins) steve.bottomley@monash.edu
Dr Noelene Quinsey (Manager of the Monash Protein Production Unit) noelene.quinsey@monash.edu
Dr Victoria Hughes (Manager of the Biophysical instrumentation) victoria.hughes@monash.edu
Facilities:
Jasco 815 Circular Dichroism Spectrometer
Malvern Zetasizer Nano-ZS
Perkin Elmer LS55B spectrofuorimeter
VP-ITC Isothermal Titration Calorimeter
Tecan Freedom Evo 200 - robotic production of proteins
Akta Avant - fast development of protein purification methods
Akta Express - high-throughput purification of proteins
Agilent Bioanalyser unit
Biorad - Gel doc EZ imager
Infors Multitron Shaker incubators (for protein expression in bacterial and insect cells)
Infors Cell Multitron shaker incubator- CO2 shaker incubator
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